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Objective To compare the genotypes distribution of human papillomavirus (HPV ) infection in tissues of cervical cancers and cervical intraepithelial neoplasias (CIN ) and its clinical significance .Methods The polymerase chain reaction (PCR) and the gene-chips technique were utilized for the detection of 23 kinds of HPV genotypes in the tissue specimens from 192 cases of cervical intraepithelial neoplasia (CIN) and 85 cases of cervical cancers .And the related data of all subjects were analyzed .Results In 192 cases of CIN ,the total positive rate of HPV was 82 .29% (158/192) ,the positive rate of single genotype infection was 46 .88% (90/192) and the positive rate of multiple genotypes infection was 35 .42% (68/192);In 85 cases of cervical cancers ,the to-tal infection rate of HPV was 88 .24% (75/85) ,the positive rate of single genotype infection was 65 .88% (56/85) and the positive rate of multiple genotypes infection was 22 .35% (19/85) .Conclusion PCR combined with the gene-chips technique can be used in the detection of the tissue samples of cervical lesions ,once detection can detect 23 kinds of HPV genotypes with high sensitivity and strong specificity ,which has very important significance to the prevention and treatment of cervical cancer and precancerous lesions and the their vaccine research .
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Objective: To study the relationship of LPPCN with neovascularization and to analyze its clini-copathologic significance, in an effort to find a new target for anti-vascular therapies. Methods: Sixty-eight ma-lignant melanoma specimens were analyzed to observe the distribution of LPPCN and to examine the expres-sion of CD105 and TGFβ1 using immunohistochemistry. The distribution of vasculogenic mimicry (VM) was observed by immunohistochemical and histochemical double staining of CD31 and PAS. Results: (1) The tu-lines and networks. Of the 68 cases of melanoma, 55.89% (38/68) were recognized as having LPPCN. (2) In malignant melanoma specimens, the rate of vasculogenic mimicry density (VMD) and microvessel density (MVD) labeled by CD105 in LPPCN-positive group were higher than those in LPPCN-negative group, with sig-nificant differences (P<0.05). VMD and MVD were positively-correlated with the density of LPPCN. The posi-tive expression of TGFβ1 in LPPCN-positive group was higher than that in LPPCN-negative group and its ex-pression in the regions of LPPCN was obviously higher than that in circumambient tumor cells, with a signifi-cant difference (P<0.05). The expression of TGFβ1 was positively correlated with MVD labeled by CD105. (3) There was no relationship between LPPCN and gender, age, site, tumor embolus, lymph node metastasis or distant metastasis (P>0.05), but LPPCN was correlated with tumor size, mitosis figure count and Breslow depth (P<0.05). Kaplan-Meier survival analysis showed the survival rate of patients with LPPCN was lower than that of patients without LPPCN, with a statistical significance (P<0.05). The presence of LPPCN indicat-ed poor prognosis. Conclusion: LPPCN exists in malignant melanoma and is associated with VM and angio- genesis. Some tumor cells undergoing LPPCN have a spacial foundation for VM and angiogenesis. LPPCN can be an index for the evaluation of the prognosis of melanoma patients.
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Objective To study the therapeutical effects and mechanisms of quercetin on hepatic fibrosis in schistosome-infected mice and compare the effects with praziquantel. Methods Eighty mice were divided into four groups: among them three groups were infected by Schistosoma japonica. After 8 weeks, one group was treated with quercetin 30 mg/(kg?d) for 8 weeks, one group was treated with praziquantel 500 mg/(kg?d) for 2 d and the other was taken as model group without any treatment. The fourth group was taken as normal group. HE staining, RT-PCR, and immunohistochemical technique were used to observe the expression of hepatic c-fos and c-jun mRNA, and the changes of hepatic TGF-?1, type Ⅰ, and type Ⅲ collagen in mice infected by S. japonica before and after treatments. Results Quercetin obviously relieved the degree of hepatic fibrosis, significantly reduced the expression of c-fos, c-jun mRNA, TGF-?1, type Ⅰ, and type Ⅲ collagen compared to the model group (P
ABSTRACT
Quercetin and praziquantel were used to treat mice with hepatic fibrosis due to Schistosoma japonicum infection.Quercetin treatment obviously relieved the degree of hepatic fibrosis,significantly reduced the expression of immediate early gene,tissue inhibitor of metalloproteinase 1(TIMP 1),typesⅠ and Ⅲ collagen compared to the control.The expression of c-jun mRNA,typeⅠ and type Ⅲ collagen were reduced significantly compared to the group treated with praziquantel,whereas no difference in the expression of c-fos mRNA and TIMP1 between the two groups,indicating that quercetin may have better effect on schistosomal liver fibrosis than praziquantel in the long term.